| Player | Pos | Seasons | Born | Birthplace | |
| Brett Oberholtzer | P | 2013-15 | Jul 1, 1989 | Christiana | DE |
| Ken Oberkfell | 3B/1B/2B | 1990-91 | May 4, 1956 | Highland | IL |
| Jim Obradovich | 1B | 1978 | Sep 13, 1949 | Fort Campbell | KY |
| Jake Odorizzi | P | 2021 | Mar 27, 1990 | Breese | IL |
| Darren Oliver | P | 2004 | Oct 6, 1970 | Kansas City | MO |
| Gregg Olson | P | 1996 | Oct 11, 1966 | Scribner | NE |
| Javier Ortiz | OF | 1990-91 | Jan 22, 1963 | Boston | MA |
| Russ Ortiz | P | 2009 | Jun 5, 1974 | Van Nuys | CA |
| Dan Osinski | P | 1970 | Nov 17, 1933 | Chicago | IL |
| Claude Osteen | P | 1974 | Aug 9, 1939 | Caney Springs | TN |
| Al Osuna | P | 1990-93 | Aug 10, 1965 | Inglewood | CA |
| Roberto Osuna | P | 2018-20 | Feb 7, 1995 | Juan Jose Rios | MX |
| Roy Oswalt | P | 2001-10 | Aug 29, 1977 | Kosciusko | MS |
| Jim Owens | P | 1964-67 | Jan 16, 1934 | Gifford | PA |
| Rudy Owens | P | 2014 | Dec 18, 1987 | Mesa | AZ |